Composite

Part:BBa_K3338013:Design

Designed by: Jonas Scholz   Group: iGEM20_Hannover   (2020-10-23)


CMV-EGFP-P2A-mCherry


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

CMV and EGFP are part of the pEGFP-C2 vector used for cloning. P2A originally originated from the porcine teschovirus 2A. The sequence of mCherry was purchased from Addgene (plasmid #45350) (Potorac et al. 2016).

References

Potorac, I., Rivero-Müller, A., Trehan, A., Kiełbus, M., Jozwiak, K., Pralong, F., Hafidi, A., Thiry, A., Ménagé, J. J., Huhtaniemi, I., Beckers, A., & Daly, A. F. (2016). A vital region for human glycoprotein hormone trafficking revealed by an LHB mutation. The Journal of endocrinology, 231(3), 197–207.